Figure 1
From: Ultra-deep sequencing of VHSV isolates contributes to understanding the role of viral quasispecies

Generation of the four overlapping amplicons (1–4) to cover the entire viral genomes of each isolate. The VHSV isolates, are here abbreviated a DK-3592b; b DK-9895174; c 1p49; d 4p168. The individual RT-PCR reactions are shown in A; B re-amplified Amplicon 3 of sample b*; C amplification of the whole genome RT-PCR reaction; D pooled amplicon samples (1:1:1:1) for each of the VHSV samples used for library preparation.