Figure 5
Growth kinetics of SDV in rainbow trout satellite cells. Primary culture of rainbow trout satellite cells were infected with SDV at an MOI of 0.1, 1 and 10 PFU per cell. Supernatant aliquots were taken at the indicated day post-infection and viral titres were determined later by indirect immunofluorescence using the anti-E2 mAb. The lower limit of detection for virus was 2 log10 PFU/mL. Each virus titration was done in duplicate. Means are shown. The standard errors were calculated, but the bars are not shown because the errors were very small.