Long-term persistence of piscine orthoreovirus-1 (PRV-1) infection during the pre-smolt stages of Atlantic salmon in freshwater

Table 1 Overview of primers used in the study.

Immune genes	Forward primer 5′–3′	Reverse primer 5′–3′	References
EF1A	CACCACCGGCCATCTGATCTACAA	TCAGCAGCCTCCTTCTCGAACTTC	[45]
MX1	GGTGGTTGTGCCATGCAA	TGGTCAGGATGCCTAATGTC	[46]
IFNG	GGTCCACTATAAGATCTCCAAGGA	CTGGCAAGATACTCCGATACAC	[46]
IL10	GCTATGGACAGCATCCTGAAGTT	GGTTGTTCTGCGTTCTGTTGTT	[47]
CD4	GTGGAGGTGCTACAGGTGTTTTC	GGGGAGGAGCCTAAAGCG	[48]
CD8a	CTTCAGCGAGGAGCAGATAAAC	GGCTGTGGTCATTGGTGTAGTC	[48]
mIgM	TGAGGAGAACTGTGGGCTACACT	TTAATGACTACTGAATGTGCAT	[47]
PRV-1	TGCGTCCTGCGTATGGCACC	R- GGCTGGCATGCCC GAATAGCA FAM-ATCACAACGCCTACCT– MGBNFQ)	[20]

ISSN: 1297-9716