Figure 1

LS or inactivated LS do not affect airway basal stem cell (BSCs) proliferation. A Schematic representation of the basal stem cells (BSCs) treatment and processing protocol. B, C Images of DAPI labelled cells in control treatment for 1 h (B) and 48 h (C). D Quantification of cell numbers in control conditions at different time points using our semi-automatic system (see materials and methods for more detail). E–H Cell number quantification at two different times and relative to control conditions in MRS medium (E), LS supernatant (F), alive LS (G) and inactivated LS (H). Scale bar in B and C represents 100 μm.