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Figure 1 | Veterinary Research

Figure 1

From: A chimeric porcine reproductive and respiratory syndrome virus 1 strain containing synthetic ORF2-6 genes can trigger T follicular helper cell and heterologous neutralizing antibody responses and confer enhanced cross-protection

Figure 1

Design, construction and rescue of the rHLJB1 and ORF2-6-CON infectious clones. This strategy was adopted from our previous studies [19, 28]. A The pACYC177-CMV-stuffer fragment containing five unique restriction enzymes was utilized for the construction of Chinese PRRSV1 full-length cDNA clones. B Four overlapping fragments were used to construct full-length rHLJB1 and ORF2-6-CON infectious clones. C The successful rescue of rHLJB1 and ORF2-6-CON was identified by IFA in PAMs and Marc-145 cells at 3 dpi, as determined by IFA. D The plaque morphology of ORF2-6-CON was determined in Marc-145 cells at 5 dpi.

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Veterinary Research

ISSN: 1297-9716