Figure 1

Derivation of neural progenitors and neurons from equine iPSC (eiPSC). A Schematic representation of the experimental procedure. B Bright field images (left) and immunofluorescence labeling of eNPCs with antibody directed against SOX2 (red, right). C, D Immunofluorescence labeling of equine brain cells from day 0 to day 14 of differentiation. C Antibodies directed against βIII-Tubulin (green) and HuC/D (red) reveal equine neurons. D Antibodies directed against SOX2 (green) and βIII-Tubulin (red) reveal eNPCs and eNe, respectively. Nuclei were stained with DAPI (blue). e: equine.