Figure 7

STING-dependent trained immunity protects mice from C. perfringens soft tissue infection. Age- and sex-matched WT and STING^−/− mice were trained with two i.p. injections of HKCA on days −7 and −4. PBS was used as the control. 7 days later, mice were intramuscularly infected with 2 × 10⁷ CFU of C. perfringens for 24 h. A Flow chart of in vivo trained immunity experimental procedure. B Representative gross images of legs. C Cumulative gross pathology scores. D Bacterial counts. Data are shown as mean ± SEM. Data were pooled from 3 independent experiments. Statistical significance is determined by *p < 0.05 and **p < 0.01.