Figure 3

miR-320 regulate target mRNA CEBPB. Marc-145 cells were transfected with miR-320 to detect the expression of the potential target mRNAs (A). CEBPB protein expression was detected by Western blot after the transfection of Marc-145 cells with miR-320 mimics or miR-320 inhibitor (B). The potential target mRNAs predicted by bioinformatics for miR-320 (C). The dual luciferase reporter assay was used to detect the luciferase activity in cells co-transfected with miR-320 mimics/inhibitor and pmirGLO-CEBPB-WT (D) or pmirGLO-CEBPB-MUT (E). Marc-145 cells were transfected with si-CEBPB, and then infected with PRRSV (MOI = 0.1). The cells were harvested at 36 h post PRRSV infection, and qRT-PCR (F) and Western blot (G) was carried out to detect PRRSV replication. All values represent the mean ± SD of three independent experiments. * p < 0.05, ** p < 0.01, *** p < 0.001.