Figure 4
From: MiR-320 inhibits PRRSV replication by targeting PRRSV ORF6 and porcine CEBPB
miR-320 inhibits PRRSV replication in vivo. Marc-145 cells were transfected with pEGFP-N1-miR-320 or pEGFP-N1 (4 µg) to detect miR-320 expression (A) and the virus copy number (B). Daily average body temperatures (C) and weight gain (D) of piglets infected PRRSV after injection with plasmid pEGFP-N1 for the positive control group and pEGFP-N1-miR-320 for the experimental group. Virus copy number (E) and CEBPB mRNA expression (F) were detected in the lungs of piglets from the experimental group and positive control group. All values represent the mean ± SD of three independent experiments. * p < 0.05, ** p < 0.01.