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Figure 6 | Veterinary Research

Figure 6

From: HSP70 positively regulates translation by interacting with the IRES and stabilizes the viral structural proteins VP1 and VP3 to facilitate duck hepatitis A virus type 1 replication

Figure 6

HSP70 and its different functional domains interact with the structural proteins VP1 and VP3. A, B Co-IP analysis of exogenous HSP70, VP1, and VP3. DEFs were co-transfected with HSP70, VP1-Flag, and VP3-Flag. After 24 h, the cell lysates were incubated with a mouse anti-HA or mouse anti-Flag antibody, and anti-HA and anti-Flag antibodies were subsequently used for immunoblotting. C, D, E, F Co-IP analysis of exogenous HA-tagged truncated HSP70, VP1, and VP3. Cell lysates were used for coimmunoprecipitation. G, H Colocalization of HSP70 with VP1 and VP3. HSP70, VP1-Flag, and VP3-Flag were transfected into DEFs. An indirect immunofluorescence assay was performed with mouse anti-HA and mouse anti-Flag antibodies and DAPI.

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Veterinary Research

ISSN: 1297-9716