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Figure 7 | Veterinary Research

Figure 7

From: HSP70 positively regulates translation by interacting with the IRES and stabilizes the viral structural proteins VP1 and VP3 to facilitate duck hepatitis A virus type 1 replication

Figure 7

HSP70 regulates the proteasomal degradation of the structural proteins VP1 and VP3. A, B Cells were co-transfected with different concentrations of HSP70-HA, VP1-Flag, and VP3-Flag, and protein expression was analyzed by Western blotting at 24 h post-transfection. C, D DEFs were transfected with VP1-Flag or VP3-Flag and incubated with VER155008 or DMSO. After 24 h, MG132 was added to the medium, and after 12 h, protein expression in the cell lysates was analyzed by Western blotting. E, F DEFs were co-transfected with HSP70-HA, VP1-Flag, and VP3-Flag, and the cells were treated with CHX (100 mg/μL) after 24 h. The cell lysates were harvested at 0, 3, 6, and 9 h, after which protein expression was measured via Western blotting. G, H DEFs were co-transfected with HSP70(1-384)-HA or HSP70(384-634)-HA and with VP1-Flag or VP3-Flag and treated with CHX. VP1 and VP3 protein expression levels were analyzed via Western blotting.

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Veterinary Research

ISSN: 1297-9716