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Table 2 Primers used for plasmid construction

From: Ursonic acid from medicinal herbs inhibits PRRSV replication through activation of the innate immune response by targeting the phosphatase PTPN1

Primer name

Sequence (5ʹ—3ʹ)

chloPTPN1-HA-F_EcoRI

CCGAATTCATGGAGATGGAAAAGGAG

chloPTPN1-HA-R_KpnI

CGGGTACCCTAAGCGTAATCTGGAACATCGTATGGGTATGTGTTGCTGTTGAACA

chloPTPN1-myc-R_KpnI

CGGGTACCCTACAGATCCTCTTCTGAGATGAGTTTTTGTTCTGTGTTGCTGTTGAACA

chloPTPN1_D181A-F

TTCCACTATACCACATGGCCCGCCTTTGGAGTCCCCGAATCGCCA

chloPTPN1_D181A-R

TGGCGATTCGGGGACTCCAAAGGCGGGCCATGTGGTATAGTGGAA

chloPTPN1_C215S-F

CACGGGCCCGTCGTGGTGCACTCCAGTGCGGGTATCGGCAGGTCT

chloPTPN1_C215S-R

AGACCTGCCGATACCCGCACTGGAGTGCACCACGACGGGCCCGTG

chloPTPN1-F_EcoRI

CGGAATTCATGGAGATGGAAAAGGAGTTCGAGCAG

chloPTPN1-R_SalI

GTGTCGACTGTGTTGCTGTTGAACAGGAACCTG

  1. chlo refers to Chlorocebus sabaeus.
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Veterinary Research

ISSN: 1297-9716