Figure 1

Mammary epithelial cells secrete sCD14. pbMEC from three different animals (red lines) and PS cells (green line) were cultured in GM medium for up to 8 days. Supernatant samples were collected at the indicated time points and used to quantify sCD14 secretion (A). At the end of the incubation, cells were detached and labelled to check for potential contamination by leukocytes producing sCD14. A dot-plot of one of the three batches of pbMEC used in this study is shown (B). Similar results were obtained with the two other batches and with PS cells. Control labeling was performed on bovine PBMC (C).