Figure 4

Heatmap and parallel plots of log(FC) for all DEG. DEG were identified using the edgeR package for the calculation of log₂(FC) and p-values. (A) log₂(FC) values against the CTRL-NONE condition were used to generate a heatmap and perform a hierarchical clustering using the pheatmap package. Four gene clusters were identified using the hclust function and are depicted on the left side of the heatmap. (B) log₂(FC) values for genes of each of the four clusters identified in A are represented as Box-whiskers plots. Box limits indicate the range of the central 50% of the data, with a central line marking the median value. Vertical lines extend from each box to capture the range of the remaining data (between 10 and 90%). Dots placed past the line edges indicate outliers. Within a cluster, log₂(FC) values for each gene in the four conditions are connected by thin lines.