Soluble CD14 produced by bovine mammary epithelial cells modulates their response to full length LPS

Table 2 Top 10 pathways identified by SPIA as differentially activated in PS cells by LPSS in the presence of CD14 compared to LPSS without CD14

KEGG pathway name	KEGG pathway ID	Size	pNDE	pPERT	pG	pGFdr	Status
Cytokine-cytokine receptor interaction	04060	144	5.45E−10	0.0001	1.72E-12	2.36E-10	Activated
Chemokine signaling pathway	04062	125	6.99E−06	0.0001	1.54E−08	1.06E−06	Activated
MAPK signaling pathway	04010	203	7.91E−08	0.02	3.37E−08	1.54E−06	Activated
NF-kappa B signaling pathway	04064	74	5.64E−07	0.02	2.18E−07	7.46E−06	Activated
Pathways in cancer	05200	259	4.41E−08	0.48	3.95E−07	1.08E−05	Activated
Amoebiasis	05146	76	3.24E−07	0.14	8.11E−07	1.85E−05	Activated
Toll-like receptor signaling pathway	04620	75	8.04E−07	0.08	1.13E−06	2.21E−05	Activated
Toxoplasmosis	05145	98	1.06E−06	0.2	3.47E−06	5.94E−05	Activated
Jak-STAT signaling pathway	04630	102	1.12E−06	0.3	5.35E−06	8.15E−05	Activated
Osteoclast differentiation	04380	90	2.34E−05	0.02	7.29E−06	9.99E−05	Activated

The list of DEG were analyzed with the SPIA package to identify, among the pathways of the KEGG database, those that are enriched in the set of DEG between LPSS-CD14 and LPSS-NONE. The table indicates the size of the pathway in the KEGG database, the probability of the significance of the given pathway Pi as provided by an over-representation analysis of the number of DEG (pNDE), the probability based on the amount of perturbation measured in each pathway (pPERT). pG is a global probability value combining pNDE and pPERT. pGFdr is an adjusted value of pG for multiple comparisons (pGFdr).

ISSN: 1297-9716