Table 1 Primer sequences used for constructing the expression plasmids
Primers | Sequence (5′ → 3′) |
|---|---|
pSTING-PF | CTAGCGTTTAAACTTAAGCTTATGCCCTACTCCAGCCTGCA |
pSTING-PR | GTCCTTGTAATCCATGCGGCCGCAGAAGATATCTGAGCGGAGTGGAA |
pcGAS-PF | CCAGATTACGCTTCGGGTACCATGGCGGCCCGGCGGGGA |
pcGAS-PR | GCCCTCTAGACTCGAGCGGCCGCCTACCAAAAAACTGGAAATCCATTG |
pSTING-K61R-PF | TAGGACTGCTGGTGCGGGGGCTCTGCAGTCTGGCG |
pSTING-K61R-PR | CCGCACCAGCAGTCCTATCTGCTGGGAGGCCA |
pSTING-K112R-PF | TCTCCATCCGAGACCGGGCTGGCCTGCCCCTCCCC |
pSTING-K112R-PR | CCGGTCTCGGATGGAGAAGTAGAAGTAGCAGG |
pSTING-K150R-PF | CTGTGAACGGAGGAACTTCAACGTGGCTCATG |
pSTING-K150R-PR | AGTTCCTCCGTTCACAGATTGCAGAGACTTCAGC |
pSTING-K187R-PF | AATCAGCGCCACCGGAACGTACTCGGGGGCATAGG |
pSTING-K187R-PR | TTCCGGTGGCGCTGATTATAAGCTTGGATCCG |
pSTING-K236R-PF | ATCCGGGGCCGGGTGTACACCAACAGCATCTA |
pSTING-K236R-PR | TACACCCGGCCCCGGATGCCAGCACGGTCGGC |
pSTING-K289R-PF | GCAGGCCCGGCTCTTCTGCCGGACCCTCGAAG |
pSTING-K289R-PR | AGAAGAGCCGGGCCTGCTCGAGCCGATCCTCC |