Table 2 Amplification of BCoV RNAs in blood samples by digital RT-PCR.
Inoculation routes | Calves | Days post-infection | Concentration | No. of partitions | Negative |
|---|---|---|---|---|---|
Positive | |||||
Intranasal | 1 | 1 | 0.000 | 0 | 25450 |
3 | 0.000 | 0 | 25456 | ||
5 | 0.000 | 0 | 25336 | ||
7 | 0.000 | 0 | 25382 | ||
9 | 0.000 | 0 | 25434 | ||
12 | 0.000 | 0 | 25433 | ||
15 | 0.000 | 0 | 25436 | ||
2 | 1 | 0.000 | 0 | 23708 | |
3 | 0.000 | 0 | 25449 | ||
5 | 0.000 | 0 | 25322 | ||
7 | 0.000 | 0 | 25451 | ||
9 | 0.000 | 0 | 25146 | ||
12 | 0.000 | 0 | 25430 | ||
15 | 0.000 | 0 | 25407 | ||
3 | 1 | 0.000 | 0 | 25440 | |
3 | 0.000 | 0 | 25446 | ||
5 | 0.000 | 0 | 25407 | ||
7 | 0.000 | 0 | 25435 | ||
9 | 0.000 | 0 | 23868 | ||
12 | 0.000 | 0 | 25442 | ||
15 | 0.000 | 0 | 25437 | ||
Oral | 1 | 1 | 0.000 | 0 | 25438 |
3 | 0.000 | 0 | 25408 | ||
5 | 0.000 | 0 | 25380 | ||
7 | 0.048 | 1 | 25387 | ||
9 | 0.000 | 0 | 23947 | ||
12 | 0.000 | 0 | 24785 | ||
15 | 0.048 | 1 | 25453 | ||
2 | 1 | 0.000 | 0 | 25448 | |
3 | 0.000 | 0 | 25453 | ||
5 | 0.000 | 0 | 25480 | ||
7 | 0.000 | 0 | 23364 | ||
9 | 0.000 | 0 | 25473 | ||
12 | 0.000 | 0 | 25217 | ||
15 | 0.000 | 0 | 25463 | ||
3 | 1 | 0.000 | 0 | 25371 | |
3 | 0.000 | 0 | 25478 | ||
5 | 0.000 | 0 | 25439 | ||
7 | 0.000 | 0 | 25418 | ||
9 | 0.048 | 1 | 25451 | ||
12 | 0.000 | 0 | 25448 | ||
15 | 0.000 | 0 | 25403 |