Figure 2

TRIM26 negatively regulates the Type I IFN signalling pathway. A and B TRIM26 inhibits poly(I:C) and SeV-triggered activation of the IFN-β promoter. HEK293T cells were transfected with IFN-β, IRF3 or ISRE reporter plasmids together with TRIM26 expression plasmid or control plasmid and increasing amounts of plasmid expressing TRIM26, and luciferase activity was analysed after transfection with poly(I:C) or infection with SeV. C and D TRIM26 inhibits poly(I:C) and SeV-triggered transcription of IFN-β and downstream genes. HEK293T cells transfected with TRIM26 expression plasmid or control plasmid were transfected with poly(I:C) or infected with SeV for 12 h, after which the expression of IFN-β, ISG15 and ISG56 was detected via qPCR. E TRIM26 deficiency enhances antiviral immunity. WT and TRIM26-KO HEK293T cells were infected with or without poly(I:C) or SeV for 12 h, and total RNA was extracted from the cells and subjected to qPCR analysis for quantitation of IFN-β, ISG15, and ISG56 in HEK293T cells. The data in A–E are expressed as the mean ± SEM of three independent experiments. **p < 0.01, ***p < 0.001; ns: not significant.