Figure 3

TRIM26 inhibits RIG-I-mediated type I IFN signalling. A PK-15 cells were transfected with plasmids expressing RIG-I, MDA5 or MAVS or infected with PRV, transfected with the IFN-β reporter plasmid and increasing amounts of the TRIM26 plasmid, and then analysed for luciferase activity. B PK-15 cells were transfected with RIG-I, MDA5 or MAVS plasmids or infected with PRV together with increasing amounts of plasmid expressing TRIM26. Total RNA was extracted from cells and subjected to qPCR analysis for quantification of IFN-β. The data are expressed as the mean ± SEM of three independent experiments; **p < 0.01, ***p < 0.001; ns: not significant.