Figure 4
From: TRIM26 facilitates PRV infection through NDP52-mediated autophagic degradation of MAVS
TRIM26 inhibits Type I IFN signalling by affecting the expression of MAVS. A PK-15 cells were transfected with the indicated plasmids (RIG-I-Myc, MDA5-Myc, or MAVS-Myc) along with control vector or TRIM26-HA expression plasmids. The lysates were analysed by Western blotting with the indicated antibodies. GAPDH was used as the protein loading control. B–D PK-15 cells were transfected with the indicated plasmids (MAVS-Myc, RIG-I-Myc or MDA5-Myc) along with control vector or increasing amounts of TRIM26-HA expression plasmids. The lysates were analysed by Western blotting with the indicated antibodies. GAPDH was used as the protein loading control. E TRIM26-HA inhibited the expression of endogenous MAVS in a dose-dependent manner. F PRV infection downregulates endogenous MAVS expression.