Figure 6
From: TRIM26 facilitates PRV infection through NDP52-mediated autophagic degradation of MAVS
TRIM26 mediates the autophagic degradation of MAVS. A Overexpression of TRIM26 had no significant effect on the mRNA level of MAVS. The mRNA levels of MAVS in PK-15 cells transfected with TRIM26 were quantitated by qRT‒PCR at the indicated time points post-transfection. The results are representative of three independent experiments. NS, not significant (p > 0.05). B TRIM26 significantly downregulated the protein level of MAVS. PK-15 cells co-transfected with MAVS-Myc and the control plasmid or with MAVS-Myc and TRIM26-HA were treated with CHX (10 μg/mL) or mock control for 0, 4, or 8 h. Western blotting was used to measure the protein levels of MAVS and TRIM26 at each time point. GAPDH was used as the protein loading control. C TRIM26 mediated the degradation of MAVS via autophagy. Western blots showing the levels of MAVS and TRIM26 in PK-15 cells co-transfected with MAVS-Myc and TRIM26-HA or empty plasmid for 16 h and then treated with DMSO, MG132, Z-VAD, 3-MA, or NH4Cl for 10 h. GAPDH was used as the protein loading control. D The relative intensity of the bands of MAVS to that of GAPDH, as analysed by ImageJ software.