Figure 6

Histopathological examinations, viral loading and viral shedding. A Histopathological examination of sheep lungs at 14 dpc. The lungs of the sheep in the vaccinated group were necropsied at 14 dpc (magnification, × 200). Lung sections from control sheep showed severely thickened alveolar septa, expansions of the alveolar interstitium, congestion, macrophage infiltration, and compensatory emphysema. The results revealed the appearance of viral pneumonia with interstitial lymphocytic infiltrates (magnification, × 200). B Formalin-fixed lung sections were subjected to OPIV3 load evaluation by IHC. C The viral load was evaluated by RT-qPCR. D Viral shedding was evaluated by RT-qPCR.