Figure 1

Anti-SADS-CoV activity of IFN-δ8 in vitro. A Analysis of IFN-δ8 by sodium dodecyl sulphate–polyacrylamide gel electrophoresis. B Analysis of the effect of IFN-δ8 on ST cells using the Cell Counting Kit-8 assay. ST cells were seeded into a 12-well plate 1 day before the experiment to form a dense single-cell layer. After 12 h of stimulation with different concentrations of IFN-δ8, the cells were infected with SADS-CoV at a multiplicity of infection of 0.01 for 24 h. C The SADS-CoV genome was evaluated by quantitative PCR. D The viral titre of the supernatant progeny was determined by the TCID₅₀. E The N protein level of SADS-CoV was determined by western blotting.