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Figure 3 | Veterinary Research

Figure 3

From: Recombinant porcine interferon delta 8 inhibits swine acute diarrhoea syndrome coronavirus infection in vitro and in vivo

Figure 3

IFITM1, viperin, Mx1, ISG15, OAS, IFIT1, and PKR expression is induced by IFN-δ8 in vivo. A IFITM1. B Viperin. C Mx1. D ISG15. E OAS. F IFIT1. G PKR. Peripheral blood mononuclear cells were isolated at the indicated time points. Total RNA was extracted using TRIzol® reagent (Invitrogen, Thermo Fisher Scientific, Waltham, MA, USA), and 1 μg of RNA was reverse-transcribed to complementary DNA. Real-time PCR was used to analyse the expression of ISGs. Relative gene expression was evaluated using the 2.−ΔΔCT method, and glyceraldehyde 3-phosphate dehydrogenase was used as an endogenous control. All the data are representative of three independent experiments (the error bars represent the standard error of the mean) and were analysed by two-way analysis of variance using GraphPad Prism software. (***p < 0.01 and ****p < 0.001 compared with the PBS control).

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Veterinary Research

ISSN: 1297-9716