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Figure 2 | Veterinary Research

Figure 2

From: Pasteurella multocida infection induces blood–brain barrier disruption by decreasing tight junctions and adherens junctions between neighbored brain microvascular endothelial cells

Figure 2

In vitro tests in human brain microvascular endothelial cell (hBMEC) models assessing the influence of Pasteurella multocida infection on hBMEC monolayers. A In vitro cytotoxicity assays revealing the conditions of hBMECs at 12-, 13-, 14-, and 15-h post P. multocida-infection. B Dextran-based transwell permeability assay showing the increase in the vascular permeability of hBMECs induced by P. multocida (PM) infection. Cells inoculated with PBS were included as control for the assay. C Transcription of tight junctions (ZO1, claudin-5, occludin) in P. multocida infected cells compared to PBS-treated cells, as determined by qPCR. D Western blots demonstrating the expression of ZO1 and E-cadherin in P. multocida infected cells and PBS-treated cells; E Quantification of western blots demonstrating the expression of ZO1 and E-cadherin in P. multocida infected cells and PBS-treated cells using ImageJ software. F Immunofluorescence images showing the reduced expression of ZO-1 in hBMECs infected with P. multocida strains compared to control PBS-treated cells. DAPI (49,6-diamidino-2-phenylindole) was used for nuclear staining.

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Veterinary Research

ISSN: 1297-9716