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Figure 3 | Veterinary Research

Figure 3

From: Pasteurella multocida infection induces blood–brain barrier disruption by decreasing tight junctions and adherens junctions between neighbored brain microvascular endothelial cells

Figure 3

Activation of the hypoxia inducible factor-1α (HIF-1α)/vascular endothelial growth factor A (VEGFA) signaling in human brain microvascular endothelial cells (hBMECs) induced by Pasteurella multocida infection. A Transcription of HIF-1α in P. multocida infected cells compared to PBS-treated cells, as determined by qPCR. B Transcription of VEGFA in P. multocida infected cells compared to PBS-treated cells, as determined by qPCR. C Western blots demonstrating the expression of HIF-1α and VEGFA in P. multocida infected cells and PBS-treated cells. D Quantification of western blots demonstrating the expression of HIF-1α in P. multocida infected cells and PBS-treated cells by ImageJ software. E Quantification of western blots demonstrating the expression of VEGFA in P. multocida infected cells and PBS-treated cells by ImageJ software. F Efficacy of specific siRNAs in suppressing the expression of HIF-1α in hBMECs post P. multocida infection; siRNA-H refers to siRNAs in suppressing the expression of HIF-1α in hBMECs. G Transcription of ZO1 in HIF-1α-knockdown cells compared to the wild type cells post P. multocida infection. H Transcription of E-cadherin in HIF-1α-knockdown cells compared to the wild type cells post P. multocida infection. I Western blots demonstrating the expression of ZO1 and E-cadherin in HIF-1α-knockdown cells compared to the wild type cells post P. multocida infection. J, K Quantification of western blots demonstrating the expression of ZO1 (J) and E-cadherin (K) in HIF-1α-knockdown cells compared to the wild type cells post P. multocida infection.

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Veterinary Research

ISSN: 1297-9716