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Figure 5 | Veterinary Research

Figure 5

From: Pasteurella multocida infection induces blood–brain barrier disruption by decreasing tight junctions and adherens junctions between neighbored brain microvascular endothelial cells

Figure 5

Influence of the NF-κB signaling on the expression of ZO1, E-cadherin, and cytokines in human brain microvascular endothelial cells (hBMECs) induced by Pasteurella multocida. A, G Efficacy of specific siRNAs in suppressing the expression of NF-κB in hBMECs post P. multocida infection; siRNA-N refers to siRNAs in suppressing the expression of NF-κB in hBMECs. B Transcription of ZO1 in NF-κB-knockdown cells compared to the wild type cells post P. multocida infection. C Transcription of E-cadherin in NF-κB-knockdown cells compared to the wild type cells post P. multocida infection. D Western blots demonstrating the expression of ZO1 and E-cadherin in NF-κB-knockdown cells compared to the wild type cells post P. multocida infection; E, F Quantification of western blots demonstrating the expression of ZO1 (E) and E-cadherin (F) in NF-κB-knockdown cells compared to the wild type cells post P. multocida infection; H–J Transcription of TNF-α (H), IL-6 (I), and IL-1β (J) in NF-κB-knockdown cells compared to the wild type cells post P. multocida infection.

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Veterinary Research

ISSN: 1297-9716