Figure 1

Antiserum preparation and IgG purification. A The purified recombinant His-TRIM5 was detected by SDS-PAGE. B The purified recombinant protein was detected by western blotting with rabbit anti-His-tag antibody as the primary antibody. C The purified recombinant proteins were detected by western blotting with prepared anti-pTRIM5 IgG as the primary antibody. D The titre of the serum was detected using ELISA.