Figure 3

TRIM5 inhibits SVA replication. A 3D4/21 cells were transfected with pEGFP-N1 or pEGFP-N1-TRIM5 for 24 h, and the expression of TRIM5 was detected by western blotting. 3D4/21 cells were infected with SVA at an MOI of 1. B The expression of the SVA VP3 gene at 6, 12, 24, 36, and 48 hpi was analysed by qRT-PCR. C, D The protein expression of VP2 at 24 hpi was detected by western blotting and quantitatively analysed by Image J. E Viral titres were determined using TCID₅₀ in PK-15 cells. F The expression of the SVA VP3 gene in adsorption and entry experiments was measured by qRT-PCR. 3D4/21 cells were transfected with shNC or shTRIM5 for 24 h. G The effectiveness of shTRIM5 was detected by western blotting. 3D4/21 cells were infected with SVA at an MOI of 1. H The mRNA expression of the SVA VP3 gene at 6, 12, 24, 36, and 48 hpi was analysed by qRT-PCR. I, J The protein expression levels of VP2 at 24 hpi were detected and analysed. K The viral titres were determined using TCID₅₀ in PK-15 cells. L The expression of the SVA VP3 gene in adsorption and entry experiments was measured by qRT-PCR. All experiments were repeated at least three times independently. ^*P < 0.05, ^**P < 0.01, ns indicates no significant difference.