Figure 4

TRIM5 increases the expression of IFNs and proinflammatory cytokines in 3D4/21 cells infected with SVA. A–E 3D4/21 cells were transfected with pEGFP-N1 or pEGFP-N1-TRIM5 for 24 h and infected with SVA at an MOI of 1. The expression of IFN-α, IFN-β, IL-1β, IL-6, and IL-18 at 6, 12, and 24 hpi was measured by qRT-PCR. F–J 3D4/21 cells were transfected with shNC or shTRIM5 for 24 h and infected with SVA at an MOI of 1. The expression of IFN-α, IFN-β, IL-1β, IL-6, and IL-18 at 6, 12, and 24 hpi was measured by qRT-PCR. All experiments were repeated at least three times independently. ^*P < 0.05, ^**P < 0.01, ns indicates no significant difference.