Figure 6

TRIM5 promotes the expression of RIG-I and RIG-I-mediated IFNs and proinflammatory cytokines. A 3D4/21 cells were transfected with different doses of pEGFP-N1-TRIM5, and the protein expression of RIG-I was detected using western blotting. B, C 3D4/21 cells were transfected with different doses of pEGFP-N1-TRIM5 and infected with SVA at an MOI of 1. The expression of RIG-I, MAVS, IRF3, IRF7, TAK1, IFN-α, IFN-β, IL-1β, IL-6, and IL-18 was measured by qRT-PCR. D The effectiveness of shRIG-I was detected by western blotting. E, F 3D4/21 cells were transfected with pEGFP-N1-TRIM5 and shNC, pEGFP-N1-TRIM5 and shRIG-I, or pEGFP-N1 and shRIG-I and infected with SVA at an MOI of 1. The expression of RIG-I, MAVS, IRF3, IRF7, TAK1, IFN-α, IFN-β, IL-1β, IL-6, and IL-18 was measured by qRT-PCR. G The mRNA expression of the SVA VP3 gene in 3D4/21 cells was measured by qRT-PCR. The experiment was repeated at least three times independently. ^*P < 0.05, ^**P < 0.01, ns indicates no significant difference.