Table 1 Primer sequences for qRT-PCR
Target Gene | Sequence (5′–3′) |
|---|---|
GAPDH | F: AAGGGCATCCTGGGCTACACTGAG |
R: TGAGGTCCACCACCCTGTTGCTGT | |
TRIM5 | F: GGAGACAAGTGAGATACGCGC |
R: CCTACCATACACCCCCAAGAT | |
SVA-VP3 | F: AACCGACCTCTTACAACTGG |
R: CTTGCCATCATAGGTCCACA | |
IFN-α | F: CTGGCACAAATGAGGAGAAT |
R: TGCTGAAGAGCTGGAAGGT | |
IFN-β | F: CTCTAGCACTGGCTGGAATGAA |
R: CCGGAGGTAATCTGTAAGTCTGTT | |
RIG-I | F: CTCGGTGGCAGATGAAGG |
R: AGCGTTAGCAGTCAGAAGG | |
MAVS | F: AGAAGCAGGACACAGAAC |
R: GAGGAGGAGGCAGTAGAC | |
IRF3 | F: GCAGGAGGACTTCGGCATCT |
R: TGGGCAGGTCGGGCTTAT | |
IRF7 | F: GACTTCGGCACCTTCTTCCA |
R: ATGGCTCCAACTTCACCAGG | |
TAK1 | F: AGGTTGTTGGAAGAGGAGCC |
R: GCCCCCTTCAGCATATTCCA | |
IL-1β | F: CGTGCAATGATGACTTTGTCTGT |
R: AGAGCCTTCAGCATGTGTGG | |
IL-6 | F: TGGCTACTGCCTTCCCTACC |
R: CAGAGATTTTGCCGAGGATGT | |
IL-18 | F: GAACCTAAACTCTCAATCATACG |
R: ATAATAAATACGGTCTGAGGTGC |