Table 2 List of primers used for qPCR.
Gene or bacterial genogroups | Organism | Primers (5′–3′) | NCBI reference sequence |
|---|---|---|---|
ef-1α Elongation factor 1-alpha | S. salar | F: CCCCTCCAGGACGTTTACAAA R: CTAAACGAAGCCTGGCTGTAAACG | NM_001123629.1 |
il-1β Interleukin 1 beta | S. salar | F: ATCACCATGCGTCACATTGC R: GTCCTTGAACTCGGTTCCCA | NM_001123582.1 |
il-8 Interleukin 8 | S. salar | F: GGCCCTCCTGACCATTACT R: ATGAGTCTACCAATTCGTCTGC | NM_001140710.3 |
il-10 Interleukin 10 | S. salar | F: ACAACAGAACGCAGAACAACC R: GCATAGGACGATCTCTTTCTTCAG | XM_045705802.1 |
tnfα Tumor necrosis factor alpha | S. salar | F: GCAGCCATCCATTTAGAGGGTGAA R: CTAAACGAAGCCTGGCTGTAAACG | NM_001123589.1 |
ifnγ Interferon gamma | S. salar | F: CTAAAGAAGGACAACCGCA R: CACCGTTAGAGGGAGAAATG | NM_001171804.1 |
Hepcidin | S. salar | F: TGTTCCTTTCTCCGAGGT R: AAAGCCACAGCCAATGT | XM_014170058.2 |
tlr5s Toll-like receptor 5 | S. salar | F: GCTGCTGGAGCTAAGGAACA R: GAGCCCTCAGCGAGTTAAGC | HQ664668.1 |
glyA Serine hydroxymethyltransferase | P. salmonis | F: CGCGTACCATTGCAGATTTCGACC R: GCTTCTAGCACACGCGGACTCG | QGP40124.1 |
LF13 | P. salmonis | F: AAAGAGCCCTGACCAAACAA R: CCCTGAGTTGTCAACAGCAA | QGO18456.1 |
EM182 | P. salmonis | F: CTCTACGCATGGGAACAGTG R: CACCACCAACAACACTACCG | QGP37999.1 |