Figure 3

Expression of DDX3X and virus replication in Marc-145 cells with distinct RK-33 treatments. A Schematic of the time-of-addition experiment of RK-33 treatments. The Marc-145 cells were infected with PRRSV Ch-1R or SD16 at an MOI of 0.1 before being treated with RK-33: pre (− 12–0 h), during (0–1 h) and post (1–48 h) virus infection. DMSO was added at the same time as a mock control. B, C DDX3X and PRRSV-N protein expressions in Marc-145 cells treated with RK-33 at different time points and infected with PRRSV were determined at 48 hpi by western blotting and RT-qPCR. D Virus titres of PRRS viruses in Marc-145 cells treated with RK-33 were detected at 48 hpi. Two-tailed Student’s t-test determined statistical analysis of comparisons, n = 3 ± SEM. The stars indicate significant differences (*P < 0.05 or **P < 0.001).