Figure 5
From: PRRSV hijacks DDX3X protein and induces ferroptosis to facilitate viral replication
Gene expression and virus proliferation in Marc-145 cells transfected with pEGFP-N1-DDX3X plasmid. A, B Cells transfected with pEGFP-N1-DDX3X or empty pEGFP-N1 were infected with PRRSV before western blotting and RT-qPCR analysis. C Marc-145 cells were infected with PRRSV Ch-1R or SD16 12-h post-transfection with pEGFP-N1-DDX3X or empty pEGFP-N1. Supernatants were then collected for TCID50 measurement. Two-tailed Student’s t-test determined statistical analysis of comparisons, n = 3 ± SEM. The stars indicate significant differences (*P < 0.05 or **P < 0.001).