Table 2 Primers for RT-qPCR amplification used in this study
From: PRRSV hijacks DDX3X protein and induces ferroptosis to facilitate viral replication
Gene | Sequence (5′–3′) | Size (bp) |
|---|---|---|
PRRSV-N | F: ATAAGAAAAGCCCGGAGAAG | 190 |
R: TGCGTCGGCAAACTAAAC | ||
DDX3X | F: TTTGCTGGCCTAGACCTGAACTC | 298 |
R: CACCTCTGTCACCACGGC | ||
β-actin | F: AGGCCCAGAGCAAGAGAGG | 463 |
R: TCACGCACGATTTCCCGC | ||
GPX4 | F: TTAGCCGCCTGTTCCGCC | 211 |
R: CATTGAGAGGCCACATTGGTG | ||
NCOA4 | F: CTTCCAACTTCGTGGTGTCC | 199 |
R: GATCACAAACTGCTGGGAGG | ||
ACSL4 | F CAAACCTGGAAGTCCATATCGC | 186 |
R: AACACCTTTCCATTTGGCTGC | ||
STEAP3 | F: GGCTCTTCGTCTGCTTCTATG | 359 |
R: CTGCTTGACTGCCAGGTTG |