Table 1 Primers or probes used in this study
Target gene | Primer/probe | Sequence (5ʹ-3ʹ) |
|---|---|---|
TGEV N | Forward | TGCCATGAACAAACCAAC |
Reverse | GGCACTTTACCATCGAAT | |
Probe | HEX-TAGCACCACGACTACCAAGC-BHQ1 | |
TNF-α | Forward | GTCTCAAACCTCAGATAAG |
Reverse | GTTGTCTTTCAGCTTCAC | |
IFN-λ3 | Forward | GCCAAGGATGCCTTTGAAGAG |
Reverse | CAGGACGCTGAGGGTCAGG | |
ISG56 | Forward | AAATGAATGAAGCCCTGGAGTATT |
Reverse | AGGGATCAAGTCCCACAGATTTT | |
IL-6 | Forward | AATGCTCTTCACCTCTCC |
Reverse | TCACACTTCTCATACTTCTCA | |
GAPDH | Forward | GGAAAGGCCATCACCATCTT |
Reverse | CATGGTCGTGAAGACACCAG | |
IFN-α | Forward | CTGCTGCCTGGAATGAGAGCC |
Reverse | TGACACAGGCTTCCAGGTCCC | |
IFN-λ1 | Forward | CCACGTCGAACTTCAGGCTT |
Reverse | ATGTGCAAGTCTCCACTGGT | |
IL-1β | Forward | AATGCTCTTCACCTCTCC |
Reverse | TCACACTTCTCATACTTCTCA | |
IFN-β | Forward | CCACCACAGCTCTTTCCATGA |
Reverse | TGAGGAGTCCCAGGCAACT |