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Figure 5 | Veterinary Research

Figure 5

From: The immunoglobulin M-degrading enzyme of Streptococcus suis (IdeSsuis) leads to long-lasting inhibition of the activation of porcine IgM-secreting B cells

Figure 5

Incubation with recombinant proteins does not impair cell viability. Porcine PBMC (n = 3, representative data points) were incubated in cell culture medium alone or with (i) rIdeSsuis_homologue (rIdeSsuis_h), (ii) rIdeSsuis_homologue_C195S (rIdeSsuis_h_C195S), (iii) rIdeSsuis_C-domain or iiii) rMRP (all 4 µg/106 cells, 45 min) before protein removal by washing. Samples were taken immediately after washing (0 h) or after 2 days or 3 days of incubation in stimulation medium (supplemented with IL-2 and R848). The cells were stained with live‒dead stain and analysed for viable cells within single lymphocytes via flow cytometry.

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Veterinary Research

ISSN: 1297-9716