Figure 6

Incubation with FITC-labelled recombinant proteins: Gating strategy. Representative pseudocolor plots are shown. Porcine PBMC were incubated in cell culture medium alone (negative control) or with FITC-labelled recombinant proteins (4 µg/10⁶ cells, 45 min). Viable cells were gated for the B-cell marker CD79α and surface IgM. Finally, the percentage of FITC⁺ IgM + B cells was analysed. The median fluorescence intensity (MFI) of IgM⁺ B cells incubated in medium alone was considered autofluorescence. The MFI of the FITC⁺ IgM⁺ B cells was considered to be specific because they were incubated with the FITC-labelled proteins.