Figure 1
From: Extracellular vesicle miRNome during subclinical mastitis in dairy cows
EV validation. Representative graphs of the results of nanoparticle tracking analysis (NTA) showing EV size distribution (A). Representative TEM images of EVs showing intact and heterogeneous EVs; top and bottom scale bars, 200 nm and 100 nm, respectively (B). Western blot analysis of EV samples. 1: isotype control antibody on EVs, 2: EVs, 3: cell lysate (A-431 cell line) (C). The EV markers used for analysis were TSG-101 (∼45 kDa), CD9 (∼24 kDa) and calnexin (∼90 kDa).