Table 1 All sequences of primers used in this experiment
From: Duck hepatitis A virus utilizes PCBP2 to facilitate viral translation and replication
Primer name | Sequence (5′-3′) | Refs. |
|---|---|---|
pCAGGS-3 × Flag-PCBP2 | GATGACGACGATAAGCTCGAGATGGACACCGGCGTCATCGA | New |
TTGGCAGAGGGAAAAAGATCTTCAGTAGGGAGAGAACCTCT | New | |
pCAGGS-3 × Flag-KH1 | GATGACGACGATAAGCTCGAGATGGACACCGGCGTCATCGA | New |
TTGGCAGAGGGAAAA AGATCTTTACGTGCTGTTGGTCATGGAGC | New | |
pCAGGS-3 × Flag-KH2 | GATGACGACGATAAGCTCGAGGCCACC ATGATCAGCAGCTCCATGACCAA | New |
TTGGCAGAGGGAAAAAGATCTTTACGCATCCAAACCTGCACTGA | New | |
pCAGGS-3 × Flag-KH3 | GATGACGACGATAAGCTCGAGGCCACCATGCCCCCGAAGGGCGTCACCAT | New |
TTGGCAGAGGGAAAAAGATCTTCAGTAGGGAGAGAACCTCT | New | |
pCAGGS-3 × Flag-ΔKH1 | GATGACGACGATAAGCTCGAGGCCACCATGGCCGCCAGCCGGCCCCCGGT | New |
TTGGCAGAGGGAAAAAGATCTTCAGTAGGGAGAGAACCTCT | New | |
pCAGGS-3 × Flag-ΔKH2 | ATGACCAACAGCACGCCCCCGAAGGGCGTCACCAT | New |
GACGCCCTTCGGGGGCGTGCTGTTGGTCATGGAGC | New | |
pCAGGS-3 × Flag-ΔKH3 | GATGACGACGATAAGCTCGAGATGGACACCGGCGTCATTGA | New |
TTGGCAGAGGGAAAAAGATCTTTACGCATCCAAACCTGCACTGA | New | |
pET-28a (+)-PCBP2 | CGCGGATCCGAATTCGAGCTCATGGACACCGGCGTCATTGA | New |
GTGGTGGTGGTGGTGCTCGAGGTAGGGAGAGAACCTCTGCT | New | |
IRES-Id + Ie | TAATACGACTCACTATATTGTGGTGGTTAGCCAACCA | New |
CAGAATGCAATTAATCCT | New | |
IRES-Ie | TAATACGACTCACTATATCTGGTGTAATGATCCCATG | New |
CAGAATGCAATTAATCCT | New | |
IRES-II | TAATACGACTCACTATAGGGGAAGGCTAGTCTATGCC | New |
CACACTCACCTACAACCT | New | |
IRES-III | TAATACGACTCACTATAGGGTGGTCTAGAGTAGGCAC | New |
AGTCTACTGGTATTATAG | New | |
PCBP2 | GGGCGCAGATCAAAATTGCC | New |
AGGAGGGTTTAGCGCTTTCT | New | |
β-actin | TACGCCAACACGGTGCTG | |
GATTCATCATACTCCTGCTTG |