Figure 1
From: RSAD2 suppresses viral replication by interacting with the Senecavirus A 2 C protein
SVA infection reduced the expression levels of RSAD2 in BHK-21 and PK-15 cells. A and B The extracted proteins and RSAD2 mRNA from SVA- or mock-infected BHK-21 cells at various time points were analysed by western blotting with anti-RSAD2, anti-VP1, and anti-β-actin antibodies (A) and reverse transcription‒quantitative polymerase chain reaction (RT-qPCR) (B), respectively. C BHK-21 cells were infected with SVA or UV-inactivated SVA for 12 h and then processed and analysed as described in panel (A) D and E Proteins (D) and RSAD2 mRNA (E) from PK-15 cells infected with SVA were analysed as described in panels A and (B) The data are expressed as the means ± standard deviations (SDs) from three independent experiments (*P < 0.05; **P < 0.01; ****P < 0.0001).