Figure 2

RSAD2 inhibited SVA replication. A and B BHK-21 cells transfected with Flag-RSAD2 or Flag plasmids were infected with SVA for 6–12 h, and the extracted proteins (A) and whole-cell culture medium (B) were then analysed. C and D BHK-21 cells transfected with Flag-RSAD2 or Flag plasmids were infected with rSVA-eGFP, and the number of rSVA-eGFP-infected cells was determined by an indirect fluorescence assay (IFA). C, and the results are presented in a histogram (D). E and F PK-15 cells transfected with Flag-RSAD2 or Flag plasmids were infected with SVA for 6–12 h, and the extracted proteins (E) and whole-cell culture media (F) were then analysed. G and H PK-15 cells transfected with Flag-RSAD2 or Flag plasmids were infected with rSVA-eGFP for 12 h, and the number of rSVA-eGFP-infected cells was then determined by IFA (G). The results are presented in a histogram (H). I and L Effects of RSAD2 silencing in BHK-21 (I) and PK-15 cells (L). J, K, M, and N BHK-21 and PK-15 cells transfected with siRSAD2 or siCon were infected with SVA for 6–12 h, and the extracted proteins (J and M) and whole-cell culture media (K and N) were then analysed. The data are expressed as the means ± SDs from three independent experiments (*P < 0.05; **P < 0.01; ***P < 0.001).