Figure 3

RSAD2 interacted with SVA 2 C. A BHK-21 cells were co-transfected with Flag-RSAD2 and various viral proteins linked with a GFP tag for 24 h, followed by fixation and incubation with anti-Flag antibodies (red signals) and DAPI (blue signals). The colocalization of RSAD2 and viral proteins was observed using confocal immunofluorescence microscopy. Scale bar, 20 μm. B HEK-293T cells co-expressing Flag-RSAD2 and GFP, GFP-L, GFP-VP1, GFP-VP2, GFP-VP3, GFP-VP4, GFP-2B, GFP-2 C, GFP-3 A, GFP-3 C, or GFP-3D for 24 h were lysed and analysed by co-IP. C and D HEK293T cells co-expressing GFP, GFP-2 C and/or Flag-RSAD2 for 24 h were lysed and immunoprecipitated with an anti-GFP or anti-Flag antibody, followed by co-IP analysis. E HEK-293T cells expressing GFP-2 C or GFP for 24 h were immunoprecipitated with an anti-GFP antibody, and endogenous RSAD2 was detected.