Figure 4

cadDX contributes to oxidative stress resistance. A Survival rates of the WT, ΔcadDX, C-cadDX, C-cadD, and C-cadX strains under H₂O₂ stress conditions. B An additional promoter of cadX inside the cadDX operon. “TTG” is the start codon of CadX. “ + 1” indicates the position of the identified additional TSS. C Volcano plot demonstrating DEGs in the ΔcadDX strain compared with the WT strain by transcriptome analysis. The cut-off for enrichment was set at |Log₂(FC)|≥ 1.0 and a p value < 0.05, as indicated by the dashed lines. The upregulated genes are marked with red dots, the downregulated genes are marked with green dots, and the DEGs within the 11K IME are marked with purple dots. Data are presented as mean ± SD, and asterisks indicate significantly different values (“*” and “****” indicate p < 0.05 and p < 0.0001, respectively).