Figure 9

Inhibition of dsRNA on the in vitro T. spiralis invasion and moulting. A larval invasion into Caco-2 monolayers after RNAi. Left: The non-invaded larvae were spirally coiled on the surface of Caco-2 cells. Scale bar: 100 μm. Right: invaded larvae were locomotive and migratory, and the integrity of the larvae-invaded Caco-2 monolayer was destroyed; the migrating trace was visible and is marked with black arrows. Scale bars: 50 μm. B larval moulting of three groups after RNAi. Larval moulting was clearly suppressed by dsRNA; no obvious sheath at the larval tail of the dsRNA-TsFATP1 group was observed, whereas clear moulting sheathes were seen in the GFP and PBS control groularvalps. Scale bars: 200 μm. * indicates P < 0.05 compared with the PBS group.