Figure 2

Identification of the cell types in EIEs by immunohistochemistry and AB-PAS staining. EIEs derived from duodenal, jejunal, and ileal tissues, as well as jejunum from a healthy horse for positive control, were immunostained (shown as brown) with Sox9 as a cell marker of intestinal stem cells, Ki-67 for proliferative cells, villin for enterocytes, chromogranin A (CgA) for enteroendocrine cells, and lysozyme (LYZ) for Paneth cells. Mucin was stained by AB-PAS as a marker of goblet cells (magenta). Black arrow heads indicate positive stained cells. Sox9-positive cells were identified throughout the EIEs. The negative control samples were not applied with primary antibodies. Scale bar = 50 μm.