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Figure 2 | Veterinary Research

Figure 2

From: Genetic characteristics associated with the virulence of porcine epidemic diarrhea virus (PEDV) with a naturally occurring truncated ORF3 gene

Figure 2

Biological characteristics of PEDV17GXCZ-1ORF3d and 17GXCZ-1ORF3c variants during serial passaging in vitro. A Plaque formation assay in different generations of the PEDV 17GXCZ-1ORF3d and 17GXCZ-1ORF3c variant strains. Vero cell monolayers were inoculated with tenfold serial dilutions of different generations of variants of PEDV strains, namely, 17GXCZ-1ORF3d and 17GXCZ-1ORF3c. After 1 h of incubation, the cells were covered with 1% agarose. The plaques were stained with crystal violet at 3–4 dpi. B Plaque diameters of different generations of 17GXCZ-1ORF3d and 17GXCZ-1ORF3c variants. The diameters of ten randomly selected patches were measured with a ruler in ImageJ 1.8.0 software. The asterisks indicate that 17GXCZ-1ORF3d-P90 and 17GXCZ-1ORF3d-P120 were significantly different from 17GXCZ-1ORF3d-P15 (**P < 0.01 and ***P < 0.001). C Viral titres of different generations of 17GXCZ-1ORF3d and 17GXCZ-1ORF3c variants in Vero cells. Vero cells were inoculated with 17GXCZ-1ORF3d or 17GXCZ-1ORF3c variants at an MOI of 0.01. The supernatants were collected at 48 hpi and measured via plaque assay for virus titration.

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Veterinary Research

ISSN: 1297-9716