Figure 3

Phylogenetic analyses of different generations of PEDV 17GXCZ-1ORF3d and 17GXCZ-1ORF3c variants on the basis of different genes. Phylogenetic trees were constructed on the basis of the aligned S protein (A), S–N gene nucleotides (B) and ORF3 protein (C) via the neighbour-joining method of MEGA-X with 1000 bootstrap replicates. Scale bars represent the branch lengths measured by the number of substitutions per site. Each PEDV strain is indicated in the following format: country of origin (three letter codes: CHN, China, JPN, Japan, KOR, Korea, MEX, Mexico, SUI, Switzerland and USA, the United States)/strain name/year of sample collection (GenBank accession number). The subgroups G1a, G1b, G2a, G2b, G2c and Group G3 are coded in red, rose red, light green, sky blue, lavender and bright yellow, respectively. The triangular symbols represent the 17GXCZ-1ORF3d and 17GXCZ-1ORF3c variants in this study, and the circles represent the reference strains of each genotype.