Figure 5

Pathogenicity analysis of the PEDV 17GXCZ-1ORF3d and 17GXCZ-1ORF3c variants. A Mean body weight changes in each group. B The average body weight changes in each group. C Fecal scores of the different groups of piglets after PEDV/mock infection. Rectal swabs were collected at different time points after PEDV infection and scored on the following criteria: 0, normal; 1, pasty stool; 2, semiliquid diarrhea; and 3, liquid diarrhea. D Survival rate of piglets in each group. E Viral RNA shedding from piglet feces after different numbers of PEDV/mock infection passages. F RNA copy number in different tissues of PEDV/mock-infected piglets. The asterisks indicate significant differences between 17GXCZ-1ORF3d-P15 and 17GXCZ-1ORF3d-P120 (*P < 0.05; **P < 0.01 and ***P < 0.001). The pound signs indicate significant differences between the 17GXCZ-1ORF3c-P15 and 17GXCZ-1ORF3c-P120 strains (^#P < 0.05).